|
A bacterial strain, producing extracellular inulin fructotransferase which converts inulin into di-D-fructofuranose 1,2´:2,3´ dianhydride(DFA Ⅲ), was isolated from soil and presumed as Bacillus sp.. The highest production of the enzyme was obtained by using medium containing Jerusalem artichoke extract as carbon source, peptone as organic nitrogen source, and NH₄H₂PO₄ as inorganic source. Under optimum condition, the enzyme activity of the culture broth supernatant reached maximal 2.61 units/㎖ after cultivation for 45 hrs.
|